Do you've got a protocol or proposed antibodies to conduct ICC staining on my fully differentiated ALI cultures?
Appraise preclinical check compounds and biologics for their qualities to modulate the immune system.
Contact us to debate personalized assay remedies for many different other cell kinds to suit your precise demands.
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This product is suitable for use in the next research place(s) as Portion of the highlighted workflow stage(s). Examine these workflows to learn more concerning the other merchandise we offer to support Each individual analysis area. Research Region Workflow Levels
For finest benefits, the overall volume of cargo added should not exceed ten% on the reaction volume. Lessening the response volume to less than eighty µL may possibly lead to reduced modifying efficiencies and cell recoveries.
Assess the status by examining a droplet and stem cell clinic stopping the dissociation when all around eighty% with the cell suspension is one-celled. Stay clear of more than-digestion.
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TEER measurements might be carried Stem cell out consistently, devoid of leading to damage to the cell lifestyle. You'll be able to perform a weekly TEER time system to describe 지방흡입 the barrier purpose during the process of ALI lifestyle differentiation.
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Use cell counts to independent the sample into two tubes – “unstained” and “with viability dye”.
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